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Airway Microbiome in Cystic Fibrosis

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What is Cystic Fibrosis?
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Lung-gut axis in CF patients
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Introduction

op October 17, 2017

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About us

op October 17, 2017

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Critical appraisal

op October 17, 2017

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What is Cystic Fibrosis?

op October 17, 2017

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Test Yourself

op October 17, 2017

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Lung microbiome

op October 16, 2017

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Lung transplantation and the lung microbiome

op October 15, 2017

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Lung-gut axis in CF patients

op October 15, 2017

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Antibiotics and airway microbiome in CF patients

op October 15, 2017

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Team Airway Microbiome: Our Weblog group comprises of five medical students attending their third year at the Leiden University Medical Centre in Leiden, The Netherlands. With our weblog each member of the group has tried to contribute to the world of the airway microbiota by writing a piece about the part of the airway microbiota which interests him or her most. The weblog writers : - Jens Krol: ‘What is cystic fibrosis?’ - Tristan Akershoek: ‘Lung microbiome’ - Malak Al-Gawahiri: ‘The impact of antibiotics on the airway microbiome of cystic fibrosis patients’ & ‘Lung-gut axis in CF patients’ - Elise van Putten: ‘Lung transplantation and the lung microbiome’ - Toon Zonneveld: ‘ Critical appraisal lower airway microbiome in children with CF With this weblog we hope to excite our readers into the world of airway microbiome as it excites us.

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About us

Our Weblog group comprises of five medical student attending their third year at the Leiden University Medical Centre in Leiden, The Netherlands. With our weblog each member of the group has tried to contribute to the world of the airway microbiota by writing a piece about the part of the airway microbiota which interests him or her most. The weblog writers : - Jens Krol: ‘What is cystic fibrosis?’ - Tristan Akershoek: ‘Lung microbiome’ - Malak Al-Gawahiri: ‘The impact of antibiotics on the airway microbiome of cystic fibrosis patients’ & ‘Lung-gut axis in CF patients’ - Elise van Putten: ‘Lung transplantation and the lung microbiome’ - Toon Zonneveld: ‘ Critical appraisal 1: airway microbiota in early CF patients’ & ‘Critical appraisal 2: lung microbiota from children with CF compared to healthy controls’ From Left to right, top: Tristan, Toon, Jens; bottom: Elise, Malak

What is Cystic Fibrosis?

Cystic fibrosis (CF) is an autosomal recessive genetic disorder which affects mainly the lungs and/or pancreas. 1 Currently, cystic fibrosis is known as the most common lethal inherited disease in the Caucasian population. 2 People with CF suffer from progressive respiratory complaints along with recurrent airway infections, but also other organ systems are impaired, which leads to malabsorption and infertility. At the moment, the prognosis for CF-patients is poor, mainly due to damage of the respiratory tract by recurrent infection which induce enormous inflammation. Since airway infections play an important role in CF, it raised the question whether this pathology can be related to the newly discovered airway microbiome. This relationship between CF and the airway microbiome will be discussed in this blog. This post will cover everything you need to know about CF to prepare you for the rest of this blog. If you are already an expert on cystic fibrosis, you can sit back and see if y

Lung microbiome

Historically the lungs have always been viewed as a sterile organ. This was later supported by bacterial cultures made from lung fluids, that turned out to be negative. When we take into consideration that bacterial based pneumonia has a 75% chance of having a negative lung culture and the fact that 70% of the human bacteria is not able to grow on standard culture media 1 , with this knowledge in mind it becomes more likely that the lung is not a sterile organ after all and actually contains it's very own microbiome. Analysis of the microbiome Rapid development in this subject area was due to the 16S ribosomal RNA exploitation in the lower respiratory tract. Almost every bacteria has rRNA which can be extracted and sequenced with modern techniques. In this rRNA a region is present that is highly specific and conserved in all bacteria. This is the 16S rRNA, which can be amplified by PCR. Samples taken by broncho-alveolar lavage (BAL) are sequenced for the 16S rRN

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